Accession Number:

AD0613083

Title:

ADENOSINE-TRIPHOSPHATASE AND POTASSIUM RETENTION IN X-IRRADIATED ERYTHROCYTES,

Descriptive Note:

Corporate Author:

ATOMIC ENERGY OF CANADA LTD CHALK RIVER (ONTARIO)

Personal Author(s):

Report Date:

1964-01-01

Pagination or Media Count:

4.0

Abstract:

An investigation was conducted to determine whether ATP-ase inhibition could account for the loss of potassium ion from x-irradiated erythrocytes. However, the loss of potassium ion from irradiated erythrocytes at low temperatures remains unexplained. It was not accompanied either by hemolysis or by loss of acid-soluble ribonucleotide bases. It was also found that the loss is reversible, for when irradiated cells 60 kr were preincubated a 4C for 20 h and then incubated at 37C for another 4 h, their potassium content increased from 26 to 66 of the original content. This re-accumulation was completely inhibited by 1 mM ouabain. It was also noted that pre-incubation at 37C for another 4 h was sufficient to prevent excess loss of potassium from irradiated erythrocytes during subsequent incubation at 4C. X-irradiation apparently destroys some component of the erythrocytes which they can repair or replace during incubation at 37C but not at 4C. However, the nature of this component has not yet been identified. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE