Accession Number:

AD0612548

Title:

SOME BIOCHEMICAL AND PHYSICAL PROPERTIES OF THE HUMAN PERMEABILITY GLOBULINS,

Descriptive Note:

Corporate Author:

WALTER REED ARMY INST OF RESEARCH WASHINGTON D C

Personal Author(s):

• Kagen,L. J.

Report Date:

1964-02-02

Pagination or Media Count:

9.0

Abstract:

The permeability activity of globulin A is inhibited by C prime 1 esterase inhibitor, soy bean trypsin inhibitor, and di-isopropylfluorophosphate. TAMe portects against this DFP inhibition, and preparations of A contain SBTI inhibitable TAMe esterase. Preparations of A are also SBTI inhibitable kininogenases which liberate a smooth muscle contracting factor from both heated 63C, 1 hr. and unheated plasma. A is estimated to have a sedimentation coefficient of approximately 11 S. It is concluded that globulin A is serum kallikrein. The permeability activity of globulin B is inhibited by C prime 1 esterase inhibitor, SBTI and DFP. TAMe protects against this DFP inhibition, and preparations of B contain SBTI inhibitable TAMe esterase. Preparations of globulin B are ineffective as kinin releasers when heated plasma 63C, 1 hr. is used as the substrate. B is estimated to have a sedimentation coefficient of approximately 6 S. It is concluded that B is PFdil. The inhibition of both permeability globulins by C prime 1 esterase inhibitor indicates that this inhibitor may be the permeability globulin inhibitor lacking in the disease, hereditary angioneurotic oedema. Author

Descriptors:

• (*GLOBULINS
• CHEMICAL PROPERTIES)
• PERMEABILITY
• INHIBITION
• CARBOXYLIC ESTER HYDROLASES
• TRYPSIN
• BLOOD SERUM
• BLOOD PLASMA
• ENZYMES
• BIOASSAY
• IMMUNITY

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE