Accession Number:

AD0611703

Title:

BLOOD LEVELS OF ACTH IN INDIVIDUAL ADRENALECTOMIZED RATS,

Descriptive Note:

Corporate Author:

CALIFORNIA UNIV LOS ANGELES

Personal Author(s):

Report Date:

1964-03-06

Pagination or Media Count:

6.0

Abstract:

Blood levels of ACTH in individual rats 3 and 10 days after bilateral adrenalectomy have been determined by the assay method of Lipscomb and Nelson, using the adrenal venous corticosteroid response of the 2-hr hypophysectomized rat. Three days after adrenalectomy ACTH levels in blood were equivalent to 0.08 0.03 mU ACTHml 10 days after adrenalectomy levels were 0.27 0.05 mUml. Pre-rinsing syringes with an acidgelatin solution prior to collection of donor blood did not significantly alter blood levels of ACTH. The ability to measure ACTH levels in the blood of individual rats 3 and 10 days after adrenalectomy supports the previous finding of an increased sensitivity of ACTH assay with the use of the 2-hr hypophysectomized rat and measurement of adrenal venous effluent corticosterone levels. The data suggest the feasibility of determining ACTH levels in blood of individual intact rats following conditions of stress. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE