ISOCITRATASE AS AN ENZYMIC INDICATOR OF METABOLIC PATHWAYS,
OXFORD UNIV (ENGLAND)
Pagination or Media Count:
Pseudomonas ovalis Chester organisms were grown on media containing respectively lysine, or the sodium salts of glutaric, acetone dicarboxylic, acetoacetic, acetic, alpha-hydroxy-glutaric, beta-ketoglutaric and glutamic acids as sole sources of carbon. The isocitratase activity of extracts of cells thus grown was measured. Growth on the first five carbon sources lysine and the substrates of path A was accompanied by high and uniform levels of isocitratase, which indicates that their catabolism led chiefly to acetate. Growth on the last three substrates the substrates of path B led to formation of only traces of the enzyme.