BASIC STUDIES IN PERCUTANEOUS ABSORPTION.
Semi-annual rept. no. 8, Jul-Dec 64,
LEVER BROS CO EDGEWATER N J
Pagination or Media Count:
Studies on the effect of organic solvents on the permeability properties of stratum corneum membranes were expanded to include the effect of sequential solvent treatments. Alterations in barrier and water-binding properties induced by differential extractions were measured and the extracts obtained analyzed in order to characterize the critical components of the corneum. In view of the probable importance of lipid-protein complexes in providing an intact skin barrier, the protein components associated with the keratinization process are being investigated. Studies on the electrodialysis of amines through stratum corneum membranes are reported, and the interaction with the barrier structure of the charged forms of the amines and the free bases is discussed. A critical comparison was made of the water diffusion rate through skin and its electrical conductivity. The results presented show a high degree of correlation, thereby demonstrating that conductivity is an excellent indicator of barrier integrity. An interesting modification of the Papanicoloau stain was developed making it possible to differentiate the various degrees of keratinization in both rat and human stratum corneum.