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THE UTILIZATION OF TRIPALMITIN-C-14 BY THE ISOLATED PERFUSED LIVER.
Final rept. for Feb 62-Jun 64,
NUCLEAR SCIENCE AND ENGINEERING CORP PITTSBURGH PA
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Research was done using isolated perfused rat liver as a method of investigating the utilization of radioactive fat emulsions. Major emphasis was placed on characterization of the fate of tripalmitin in different emulsion preparations introduced to control livers, irradiated livers and starved livers in order to achieve a better understanding of the utilization of the lipid in fat emulsions suitable for use in intravenous nutrition. Results obtained with the isolated perfused rat livers were found to be similar to observations of fat metabolism observed in intact animal studies. Elevations of labeled triglycerides and phospholipids in the perfusates of irradiated livers exposed to both radioactive Lipomul and SR Emulsions were observed to be not unlike the post-irradiation hyperlipemia previously reported. A similar situation was observed in starved liver experiments. Evidence was obtained that irradiated and starved livers utilize other lipid components of cottonseed oil more readily than the tripalmitin content. Tripalmitin-C-14 appeared to follow the same fate regardless of whether it was administered in a Lipomul Emulsion or a SR Emulsion except that labeled phospholipids did not appear to be released from the liver to the perfusate when SR Emulsion was employed. It is suggested that the emulsifiers in the SR Emulsion may influence the fate of phospholipids derived from tripalmitin. Author
APPROVED FOR PUBLIC RELEASE