ENZYMATIC STUDIES ON THERMOPHILIC BACTERIA
Final rept. for 1 Feb 1962-31 Dec 1964
ILLINOIS UNIV AT URBANA
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The objectives of the research were 1 to prepare crystalline alpha-amylase from thermophilic bacteria and to study the physical and chemical properties of the preparations in order to obtain data relative to the problem of thermal stability 2 to study the biosynthesis of alpha-amylase by Bacillus stearothermophilus 3 to study conditions of induction of a lysogenic system in B. stearothermophilus 4 to isolate and purify enzymes from thermophilic bacteria related to amino acid and carbohydrate metabolism and 5 to study the physical and chemical properties of the above enzymes. Studies were initiated on the sequence of the alpha-amylase of B. stearothermophilus by using chains A and B which are formed by performic acid oxidation. Conditions for the isolation of peptides were examined and efforts were made to obtain sufficient material to study the sequence of the two chains.