CHEMICAL FACTORS IN CEREBRAL EXCITATION.
Final technical rept.
MAUDSLEY HOSPITAL LONDON (ENGLAND)
Pagination or Media Count:
Membrane potentials recorded intracellularly from slices of cerebral cortex gave a mean value of 59 10 mV S.D.. Potentials were more stable in media containing 2.6 mM calcium than in media with 0.75 mM calcium. Small amounts of KCl were applied to the tissue surface, and calculated to give by diffusion concentrations of K up to 100 mM near the cells under observation. The KCl diminished the potentials, which subsequently recovered. Similar results were obtained by additions of sodium glutamate. Analysis of the tissue for Na, K, Cl and inulin showed that considerable gradients existed for these ions between tissue and incubation fluid the gradients were compared with the observed membrane potentials. Author