Accession Number:

AD0600255

Title:

SPECTRAL STUDIES OF THE PHOTOBLEACHING OF RIBOFLAVIN PHOSPHATE.

Descriptive Note:

Special technical and scientific rept. no. 2,

Corporate Author:

UPPSALA UNIV (SWEDEN)

Personal Author(s):

Report Date:

1964-04-01

Pagination or Media Count:

35.0

Abstract:

The spectral changes during photobleaching of riboflavin phosphate Rf in neutral solution was studied, both in oxygen free solutions, in presence of small amounts of oxygen, in air saturated solutions, and in presence of hydrogen peroxide. After anaerobic photolysis, aeration, and extraction of lumichrome Lc the solution contains an alkali sensitive and extremely light sensitive compound, deuteroflavin Df. It was proved that these properties can not be due to hydrogen peroxide. The destruction of Rf takes place during the illumination and not during the aeration. After an initial build-up period, the anaerobic photolysis is a steady state process with regard to leuco-deuteroflavin H sub 2 Df. Small amounts of oxygen increase the yield of Lc and decrease the concentration of H sub 2 Df. Aerobic photolysis leads to Lc and a compound with an absorption macimum at 400 millimicrons. Similar results are obtained in presence of H sub 2 O sub 2. Previous observations regarding the decrease in the quantum yield during the course of the anaerobic photobleaching, and the buffer effect, are confirmed, although not quantitatively. The results are compared with those from experiments with 10 mM EDTA.

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE