PHARMACOLOGICAL STUDIES ON IRRADIATED ANIMALS. PART I. SCOPE AND METHODOLOGY.
NAVAL MEDICAL RESEARCH INST BETHESDA MD
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The scope of pharmacological studies on irradiated animals has been discussed and the necessity for clarification of procedures in experimental studies in this field has been pointed out. As two main lines of experimental approach in studies utilizing the lethal effect of X-rays in total body irradiation, the following have been suggested 1 use of various drug concentrations on one X-ray dose, preferably a dose close to the LD5014 days for the particular animal species and 2 the use of one drug concentration on at least two radiation doses, one of them the mid-lethal, the other of either the low-lethal, or high-lethal range. The procedures are exemplified by presentation of preliminary data concerning the effects of desoxyribonucleic and ribonucleic acid as well as those of a spleen extract on the radiation induced mortality in mice. The necessity of utilizing the entire lethal dose curve for the evaluation of drug effects has been demonstrated. Supplementary methods for further elucidation of the mechanisms by which pharmacological agents modify the lethal effect of ionizing radiation consisting in detailed histological and bio-assay studies of isolated organs have been pointed out.