Accession Number:

AD0485846

Title:

A RAPID AND SIMPLE PHOSPHOLIPASE A ASSAY,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s):

• Kocholaty, Walter F.

Report Date:

1965-12-30

Pagination or Media Count:

7.0

Abstract:

A simple and rapid method for the assay of phospholipase A was developed. As a substrate fresh egg yolk is used which is hydrolyzed by snake venom phospholipase A at a 10-20 x faster rate than pure lecithin. The released fatty acids, after extraction with appropriate solvents are titrated automatically. The performance of the electrodes in non-aqueous titrations is discussed. The described method will permit a great number of fast assays of phospholipase A, and the titration of fatty acids is free from subjective errors since it is carried out automatically. Author

Descriptors:

• (*PHOSPHORIC MONOESTER HYDROLASES
• MICROANALYSIS)
• TEST METHODS
• VENOMS
• HYDROLYSIS
• EGGS
• FATTY ACIDS
• PURIFICATION
• VOLUMETRIC ANALYSIS

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE