Accession Number:
AD0485846
Title:
A RAPID AND SIMPLE PHOSPHOLIPASE A ASSAY,
Descriptive Note:
Corporate Author:
ARMY MEDICAL RESEARCH LAB FORT KNOX KY
Personal Author(s):
Report Date:
1965-12-30
Pagination or Media Count:
7.0
Abstract:
A simple and rapid method for the assay of phospholipase A was developed. As a substrate fresh egg yolk is used which is hydrolyzed by snake venom phospholipase A at a 10-20 x faster rate than pure lecithin. The released fatty acids, after extraction with appropriate solvents are titrated automatically. The performance of the electrodes in non-aqueous titrations is discussed. The described method will permit a great number of fast assays of phospholipase A, and the titration of fatty acids is free from subjective errors since it is carried out automatically. Author
Descriptors:
Subject Categories:
- Biochemistry