THE BIOLOGICAL SIGNIFICANCE OF PROTEOLYSIS IN INFLAMMATION, WITH SPECIAL REFERENCE TO THE ROLE OF A SPECIFIC PROTEASE INHIBITOR ISOLATED FROM ANIMAL TISSUE.
Rept. no. 8 (Final). 1 Jun 65-31 May 66,
KUMAMOTO UNIV (JAPAN) MEDICAL SCHOOL
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The Arthus permeability factor PF mediating the Arthus delayed vascular response, the major event in the response, was newly isolated from the local site and highly purified by column chromatography. The PF was not a single chemical entity, but it may be a series of PFs, i.e., Arthus PFs I, II and III, which may be of polypeptide in nature and the prolonged delayed vascular response seemed associated with the functional successions of these PFs. The Arthus PF II was most active and obtained as a homogenous substance electrophoretically. It seemed possible that these PFs were correlated with the activity of Arthus protease but this remained to be explored. However, it was clearly confirmed that the anaphylactic release of the immediate PF or histamine, mediating the Arthus immediate vascular response, was caused by activation of the Arthus protease in the antigen-antibody reaction induced cells, because the release was greatly decreased by a specific polypeptide inhibitor. The Arthus PF was inactivated by a specific antifactor newly isolated from decreased vascular lesion it was extracted in euglobulin fraction and was a proteolytic enzyme, different from Arthus protease. The effect paralleled its proteolytic activity. Similar PFs and antifactor were isolated from thermal vascular lesion, respectively, and purified highly. Author
- Medicine and Medical Research