Accession Number:

AD0483823

Title:

FORMAL PROGRESS REPORT.

Descriptive Note:

Rept. for period ending 30 Apr 66,

Corporate Author:

MASON RESEARCH INST WORCESTER MA DEPT OF IMMUNOLOGY

Personal Author(s):

Report Date:

1966-04-30

Pagination or Media Count:

19.0

Abstract:

The two primary of major concern were the developments of an affective isimmunization procedure, and a sensitive and repeatable serological test system for determining the reactivity of rhesus isantisera. Since histocompatibility antigens may be shared by both erythroctes and leukocytes, serological methods involving both cell types were studied. Of greatest concern was the isoimmunization protocal to be employed in the rhesus animal. The possibility that the isoimmune response to intracutaneous injection of leukocytes might be limited to skin sensitivity or the regin type of reaction was investigated. The strictly intracutaneous route was modified to include an injection of leukocytes into the palm of the left hand. The hypothesis was that lymphatic drainage from an extremity would be relatively rapid to the regional lymph nodes, and the sensitization resulting as a consequence of intracutaneous injection, would be further stimulated to result in the production of bivalent leukoagglutinating and hemagglutinating antibodies. Whatever the validity of this hypothesis, the results of the isoimmunizing protocol used in these studies has been excellent. Both leukoagglutinating and hemagglutinating antibodies have been induced, and the reactions are strong and repeatable in in vitro test systems.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE