Accession Number:

AD0483346

Title:

A STUDY OF IMMEDIATE SENSITIZATION BY ADSORPTION OF ANTIGENS AND ANTIBODIES IN VITRO

Descriptive Note:

Annual progress rept.

Corporate Author:

STANFORD UNIV CA SCHOOL OF MEDICINE

Personal Author(s):

Report Date:

1966-05-16

Pagination or Media Count:

95.0

Abstract:

Studies on the kinetics of sensitization have shown that the rate attains a maximum and depends on the antibody concentration and temperature. The lag period varies inversely as the temperature and inversely as the logarithm of antibody concentration. Sea Urchin Toxin The active fraction is a proleolytic enzyme which attacks alpha 2-globulins to produce a dialyzable kinin Streptolysin. Experiments on atrial action potentials show that the repolarization rate of single cells is increased by streptolysin, suggesting that one of the effects of the toxin is on the Ach-ChE system of the heart. Tetanus We have shown that the two physiological activities in crude tetanus toxin are properties of different molecules present in the mixture. A partial separation has been effected and the physical properties of the two factors have been determined 1 Purification of the MEPP factor by the combined treatment of the crude preparation with ammonium sulfate and cerebroside-ganglioside enriches the electrophysiological potency 200-fold and decreases the lethal toxicity half a million-fold. 2 Partial purification of the tetanospasmin by methanol precipitation decreases the MEPP factor 90,000-fold and increases the lethal toxicity 6-fold. 3 The two factors can be separated immuno- electrophoretically. 4 The activity of the MEPP factor is concentrated with the 4S group of proteins and that of the lethal factor with the 7S proteins. 5 Estimates of the molecular weight show that the lethal factor has a value of 176,000 and that the MEPP factor has a value of about 59,000.

Subject Categories:

  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE