ANTIBODY FOR PSITTACOSIS GROUP AGENTS IN ASCITIC FLUIDS OF MICE IMPLANTED WITH SARCOMA 180
ARMY BIOLOGICAL DEFENSE RESEARCH CENTERFREDERICK MD
Pagination or Media Count:
Preparation of neutralizing antisera to psittacosis group agents in laboratory animals is difficult, and antisera to these agents are available in limited quantity. Methods for the production of immune ascitic fluids in mice suggested a means of obtaining antisera for these agents. Mice were given three intraperitoneal injections of formalinized vaccines prepared from either the Borg or 6BC strains of the psittacosis group agents and challenged by the same route 3 weeks after the last injection. Sarcoma 180 cells were implanted in the surviving mice 2 weeks after challenge, and ascitic fluids 20 to 30 mlmouse were collected at 1- to 2-week intervals thereafter. Borg immune ascitic fluid obtained through day 29 neutralized greater than 100,000 MIPLD sub 50 and neutralizing activity was demonstrable up to 132. The neutralizing capacity of the 6BC immune ascitic fluid was much lower approximately 1,000 to 10,000 MIPLD sub 50 were neutralized. Passive protection with Borg immune ascitic fluid was strongly evident on the 5th day following intraperitoneal immunization and had disappeared by the 15th day.
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- Medicine and Medical Research