Accession Number:

AD0478434

Title:

HYDROXYAPATITE CHROMATOGRAPHY OF BACILLUS SUBTILIS NUCLEIC ACIDS,

Descriptive Note:

Technical manu.

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s):

• Mendelson, Neil H.

Report Date:

1966-01-01

Pagination or Media Count:

11.0

Abstract:

Chromatography of Bacillus subtilis nucleic acids on hydroxyapatite has been examined. Newly synthesized nucleic acids were labelled during spore germination by P32 incorporation. Nucleic acids were eluted from hydroxyapatite columns by passing an increasing linear phosphate molarity gradient through the columns. Three peaks were obtained. These peaks 1,2, and 3 were examined in detail using a variety of techniques. From these data it may be concluded that peak 3 is newly synthesized native B. subtilis DNA, and peak 2 is newly synthesized RNA. Peak 1 appears to consist of low molecular weight DNA fragments of unusually heavy buoyant density in CsC1. It is suggested that peak 1 fragments come from the replicating points of the DNA molecule near the origin.

Descriptors:

• *BACILLUS SUBTILIS
• *NUCLEIC ACIDS
• SPORES
• DEOXYRIBONUCLEIC ACIDS
• MOLECULAR PROPERTIES
• BIOSYNTHESIS
• LABELED SUBSTANCES
• CHROMATOGRAPHIC ANALYSIS
• GERMINATION

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE