SOME CHARACTERISTICS AND KINETICS OF COAGULASE RELEASE BY STAPHYLOCOCCUS AUREUS
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
Coagulase is realeased from growing cells of strains of Staphylococcus aureus when purified bovine serum albumin is added to the medium. When cells are grown in albumin-free medium to an optical density of 0.2 and then resuspended in media containing albumin, two types of responses are noted some strains show an immediate and steady rate of release of coagulase, others show a considerable 40 to 60 min lag followed by release of coagulase at a much lower but also steady rate. Coagulase is not released by resuspension in albumin solution alone. Horse, human, bovine, and porcine albumins elicit the same rate of coagulase release, but egg albumin promotes coagulase release at a considerably lower rate. Bovine hemoglobin, gelatin, transferrin, and bovine alpha, beta, and gamma globulins do not promote the release of coagulase. Slightly acid pH suppresses release of coagulase from cells growing in media containing albumin. Proper concentrations of chloramphenicol completely prevent elaboration of coagulase but allow unaltered cell growth. Cell-free extracts prepared by two methods failed to show any intracellular coagulase.