Accession Number:

AD0472301

Title:

RAPID QUANTITATIVE SEROLOGICAL ASSAY OF STAPHYLOCOCCAL ENTEROTOXIN B

Descriptive Note:

Technical manuscript

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s):

• Weirether, Francis J.
• Lewis, Evelyn E.
• Rosenwald, Albert J.
• Lincoln, Ralph E.

Report Date:

1965-09-01

Pagination or Media Count:

25.0

Abstract:

A simple, rapid method, based on the Oudin single gel diffusion technique, is described for the quantitative assay of staphylococcal enterotoxin B. The method yields reproducible results without close control of such assay variables as temperature, antiserum dilution, and assay time, provided that the ionic strength is maintained above 0.2 N sodium chloride equivalent.

Descriptors:

• *BACTERIAL TOXINS
• *STAPHYLOCOCCUS AUREUS
• BUFFERS
• IONS
• TEMPERATURE
• QUANTITATIVE ANALYSIS
• PRODUCTION
• BIOCHEMISTRY
• SODIUM COMPOUNDS
• CULTURE MEDIA
• ANTIBODIES
• DIFFUSION
• IMMUNE SERUMS
• GELS
• ANTIGENS

Subject Categories:

• Toxicology
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE