Accession Number:

AD0472299

Title:

BACILLUS SUBTILIS PHAGE SP-10: CONCURRENT CHANGES IN TRANSDUCING EFFICIENCY AND CONTENT OF TRANSFORMING DEOXYRIBONUCLEIC ACID,

Descriptive Note:

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s):

Report Date:

1965-09-01

Pagination or Media Count:

24.0

Abstract:

Spores of Bacillus subtilis infected with transducing phage SP-10 served as convenient inocula for broth cultures from which transducing phage was harvested. Methods are described for producing highly infected spores. The inoculum level of infected spores in nutrient broth yeast-extract glucose medium affected the transducing efficiency of SP-10 in lysates of these cultures. The combination of inoculum level and incubation time required to produce the best transducing phage had to be determined empirically for each batch of infected spores. Several possible explanations for the difference between lysates having high HTE and those having low LTE transducing efficiency were ruled out by special experiments. The hypothesis is presented that some cultural condition resulting from a relatively low inoculum of phage-infected spores favors the incorporation by phage particles of bacterial deoxyribonucleic acid DNA in the manner required for the production of transducing phage. Support for this hypothesis is a demonstration, through transformation experiments with DNA extracted from HTE and LTE phage particles, that populations of HTE phage particles yielded significantly more 7 to 27 times transforming activity per microgram of DNA than did populations of LTE phage. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE