HEMAGGLUTINATION-INHIBITION METHOD AND IMMUNOFLUORESCENCE STAINING WITH VEE VIRUS
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
Hemagglutination and fluorescent antibody are compared for the direct detection of virus devoid of host cells. The minimum number of tissue plaque- forming units of Venezuelan equine encephalomyelitis virus that could be detected by the hemagglutination technique was determined. Similar concentrations of the virus in bovine albumin borate saline, brain-heart infusion broth, and demineralized water were tested by the fluorescent antibody technique. Somewhat higher concentrations of the virus in bovine albumin borate saline were used in the hemagglutination-inhibition test. The quantitative hemagglutination procedure employed for these studies was carried out at 37 C for 75 minutes with variations in concentration of goose red cells. As a result of lowering the red cell concentration, smaller concentrations of virus were detected. The direct fluorescent antibody staining procedure applied to slide preparations containing known numbers of tissue culture plaque-forming units of virus was negative. Adsorbed viral antigen on agglutinated goose erythrocytes was visualized by direct and indirect FA techniques.