RED CELL METABOLISM RELATED TO BLOOD STORAGE.
Annual progress 1 Jul 64-30 Jul 65,
LABORATORY FOR COMPARATIVE BIOCHEMISTRY SAN DIEGO CA
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A detailed study has been carried out on the metabolism of the deoxyribose analogs of inosine and adenosine by outdated blood-bank stored human red cells. Both compounds are utilized rapidly to give hypoxanthine, acetaldehyde, lactic acid and a variety of intracellular intermediates including deoxyribose 5-phosphate, deoxy xylulose l-phosphate and deoxy octulose 1, 8-diphosphate, in addition to the usual glycolytic intermediates. Deoxyadenosine but not deoxyinosine or deoxyinosine plus adenine led to the synthesis of considerable amounts of deoxyATP. There was evidence for the conversion of deoxyribo nucleotide to ribo nucleotide. Evidence is brought forward that a compound identified as ribose 1, 5-diphosphate which accumulates in human red cells incubated with inosine but not with inosine plus adenine, is in fact a degradation product of 5-phosphoribosyl 1-pyrophosphate. An improved method is reported for the recovery by ion-exchange column chromatography from red cell incubations of inosine, adenosine, deoxyinosine, deoxyadenosine, hypoxanthine and adenine. Author