Accession Number:

AD0468159

Title:

IMMUNOLOGICAL STUDIES OF ANTHRAX: 4. EVALUATION OF THE IMMUNOGENICITY OF THREE COMPONENTS OF ANTHRAX TOXIN

Descriptive Note:

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Report Date:

1965-07-01

Pagination or Media Count:

25.0

Abstract:

Three components of anthrax toxin edema factor, protective antigen, and lethal factor were separated and tested singly at three dose levels and in factorial combination 27 treatments to determine their efficacy as immunogens in a resistant host rat and in a susceptible host guinea pig. The efficacy of each component used as an immunogen was determined by its ability to 1 protect against the establishment of the disease, 2 influence the number of bacilli-ml of blood at death, 3 change the units of toxinml of blood at death, 4 affect the antibody titer Ouchterlony, and 5 alter the units of toxin neutralizedml of blood. Lethal factor was the only immunogenic component against both R. anthracis spore and toxin challenge in each of the hosts tested. Protective antigen was protective in the guinea pig as both a primary and interactive response. In both animals the edema factor was nonimmunogenic by the methods of evaluation and gave poor protection against both the spore and toxin challenge however, certain interactions were noted. The interaction of antibody and the dual defense mechanisms in resistant and susceptible hosts are discussed.

Subject Categories:

  • Medicine and Medical Research
  • Toxicology
  • Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE