STUDIES OF METABOLISM AND MECHANISMS OF DESTRUCTION OF THE FORMED ELEMENTS OF THE BLOOD.
Annual progress rept.,
ROCHESTER UNIV NY SCHOOL OF MEDICINE AND DENTISTRY
Pagination or Media Count:
Na acetate 1 Cl4, given intravenously, produces measurable labelling of plasma phospholipids and cholesterol within 24 hours in the dog. Analysis of the exchange of plasma and erythrocyte lipids using this isotope suggest that only 85 of the red blood cell cholesterol is in equilibrium with plasma cholesterol, and that the exchange of lecithin between plasma and red blood cells involves the entire molecule as a unit. The incorporation of extracellular orthophosphate P32 into erythrocyte ATP, inorganic phosphate and 2,3-DPG has been measured at 12, 1, 2, 4, and 6 hours, in vitro at 37 C, in five patiens with Hereditary Spherocytosis H.S. and five normal subjects. No difference between the two groups was found in the rate or degree of such incorporation. The lipid composition of the erythrocytes from seven patients with H.S. has been studied and found normal. During in vitro incubation at 37 C for 24 hours, H.S. erythrocytes lose about 22 percent of their membrane lipid when no glucose is added. Glucose kiminishes this loss of lipid but does not prevent it completely. Oqubain has no effect on lipid loss. Under comparable conditions normal erythrocytes lose little or no lipid. Author