Accession Number:

AD0466947

Title:

EFFECT OF ENZYMATIC FUNCTIONAL GROUP INHIBITORS ON THE ESTERASES OF CROTALUS ATROX VENOM,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s):

• Brown, John H.

Report Date:

1965-04-23

Pagination or Media Count:

10.0

Abstract:

The activities of phosphodiesterase, phosphomonoesterase and esterase in Crotalus atrox venom can be completely inhibited by one or more of the following enzymatic functional groupspecific reagents 2-mercaptoethanol, 2,3dimercaptopropanol, p-hydroxymercuibenzoate, iodoacetamide, diisopropylfluorophosphate, ethylenediaminetetraacetic acid, N-bromosuccinimide. Phosphodiesterase was activated by the divalent cations of iron, cobalt, nickel, manganese, zinc and the alkaline earths phosphomonoesterase was activated by calcium, iron, cobalt and zinc esterase was not activated by metal ions. In general, the divalent cations of cadmium, mercury and copper inhibited all three enzymes. Author

Descriptors:

• *ENZYMES
• BIOCHEMISTRY
• VENOMS
• ORGANIC PHOSPHORUS COMPOUNDS
• REPTILES
• CARBOXYLIC ESTER HYDROLASES
• PHOSPHORYLATION
• TOXICITY
• PH FACTOR
• HYDROLYSIS
• ORGANOMETALLIC COMPOUNDS
• IONS
• ALKALINE EARTH METALS

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE