DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click HERE
to register or log in.
TRANSFER OF F-LAC EPISOME TO PASTEURELLA PSEUDOTUBERCULOSIS
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The F-lac episome was transferred from Escherichia coli to Pasteurella pseudotuberculosis. The parent P. pseudotuberculosis fermented glycerol, rhamnose, salicin, and xylose, produced urease, VW antigens, and glucose-6-phosphate dehydrogenase, was pigmented, pesticin-sensitive, and virulent, and had a calcium requirement at 37 deg C. Some of the P. pseudotuberculosis F-lac recipients were negative for all of these properties, but became positive for all of them again when the F-lac episome was lost. It appeared that the F-lac carried with it a genetic unit that could repress the expression of several seemingly unrelated properties, and that this repression was removed when the episome was lost.
APPROVED FOR PUBLIC RELEASE