Accession Number:

AD0453592

Title:

NUCLEIC ACID INTERACTIONS. V. EFFECTS OF CYCLOBUXINE,

Descriptive Note:

Corporate Author:

INDIANA UNIV BLOOMINGTON

Personal Author(s):

Report Date:

1964-07-07

Pagination or Media Count:

19.0

Abstract:

In aqueous media of low ionic strength Na 0.006 M the alkaloid cyclobuxine, a steroidal diamine, exerts a profound effect on the stability of helical polynucleotides DNA, S-RNA and dATdAT. This effect is biphasic in the sense that at low concentrations of the agent or 0.0001 there is stabilization of the native, while ac higher concentrations or 0.0005 there is stabilization of the denatured conformation. In a medium containing 51 methanol only the labilizing interaction persists while at high ionic strength both types of interaction are eliminated. These effects of cyclobuxine are restricted to the reversible order-disorder transition and do not extend to strand separation or re-combination. Cyclobuxine effects the absorption spectrum of DNA and other polynucleotides by producing a generalized hyperchromicity centered at 270 millimicrons. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE