RELATIONSHIP BETWEEN COMPOUND STRUCTURE AND INFECTIVITY EFFECT ON EEE VIRUS
VICKERS-ARMSTRONGS (AIRCRAFT) LTD WEYBRIDGE (UNITED KINGDOM)
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A number of sulfhydryl -SH compounds and the nonsulfur analogs of some of these compounds were tested for their effect on the infectivity of partially purified eastern equine encephalitis virus during storage at 4 C. Thiourea was found to be the most effective stabilizer whereas urea and guanidine caused a loss of viral infectivity. Thiosemicarbazide and semicarbazide showed effects similar to those of thiourea and urea. Viral inactivation produced by p- chloromercuribenzoate, sodium thioglycollate, or sodium ascorbate could be prevented by thiourea. No correlation was found between the oxidation-reduction potential of virus suspensions containing the various compounds and the infectivity titer, or change in titer, of these suspensions. The presence of an -SH group in the molecule is not a requisite for stabilization glutamine and dipyridyl exhibited good stabilizing properties.
- Medicine and Medical Research