GROWTH CHARACTERISTICS OF PSITTACOSIS GROUP AGENTS IN HUMAN DIPLOID CELLS
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
A comparison was made of the growth and morphological development of two agents of human pneumonitis strains Borg and SF and one agent of psittacosis strain 6BC in one cell line L cells and several commercially available HDCS, including lung, skin and muscle, kidney, and whole embryo strains. Growth of the three agents was supported by each of the diploid cell strains in all combinations that were tested, although the three agents were not tested in each strain. Results obtained by growing the agents in HDCS were essentially the same as those obtained in the L cell line. A cytopathic effect CPE described as floating cellular debris, rounding of cells, holes, and a granular appearance throughout parts of the cell monolayer was observed in all cell cultures and with each of the agents used. The two strains SF and Borg of the human pneumonitis agent differed in growth pattern, CPE observed in cell cultures, and the type of inclusion bodies formed in human lung cells. The SF agent resembled more closely the 6BC strain of the psittacosis agent. Comparative titers, cytopathology, and morphological development of the agents in HDCS are discussed.