Accession Number:

AD0442462

Title:

THE METABOLISM OF ACETATE BY WHEAT STEM RUST UREDOSPORES,

Descriptive Note:

Corporate Author:

NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB

Personal Author(s):

Report Date:

1963-12-30

Pagination or Media Count:

6.0

Abstract:

When uredospores of Puccinia graminis var. tritici race 15B were incubated at pH 6.2 in phosphate buffer containing either acetate-1-C14 or 2-C14, about 12 of the radioactivity was removed from the solution in a period of 3 hours. Respired carbon dioxide contained about 45 and 22 of the carbon-14 taken up as acetate-1-C14 and acetate-2-C14, respectively. Incorporation of carbon-14 into spore components was considerably higher with acetate-2-C14 than with acetate1-C14. With either tracer most of the radioactivity in water-soluble spore materials was accounted for in amino acids and neutral substances. Glutamic acid was particularly radioactive and accounted for about 40 of the radioactivity in the amino acid fraction. Incorporation of carbon-14 into the glutamic acid skeleton was consistent with the view that both the tricarboxylic acid cycle and the glyoxalate cycle were functioning. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE