Accession Number:

AD0442409

Title:

STUDIES ON THE PURIFICATION OF VACCINIA VIRUS

Descriptive Note:

Technical Manuscript

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s):

• Orlando, Michael D.
• Riley, Jean M.
• Patrick, III, William C.

Report Date:

1964-05-01

Pagination or Media Count:

15.0

Abstract:

Various methods are described and compared for removing extraneous matter from vaccinia virus suspensions. Of ten procedures tested, differential centrifugation and Freon extraction were the most successful. Both the partially purified suspensions and the dried products prepared from them had high virus titers in embryonated eggs and high infectivity for mice. Although both processes resulted in products with the same titers, the differentially centrifuged product was approximately tenfold more purified as shown by the percentage solids of the suspensions before freeze drying.

Descriptors:

• *PURIFICATION
• *VACCINIA VIRUS
• FREEZE DRYING
• EXTRACTION
• EMBRYONATED EGG TECHNIQUE
• MICE
• INFECTIOUS DISEASES

Subject Categories:

• Pharmacology
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE