ELECTRON MICROSCOPIC AND IMMUNOFLUORESCENT OBSERVATIONS ON YELLOW FEVER INFECTION
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
Immunofluorescence and electron microscopy were used to study changes in liver cells of cynomolgus monkeys and tissue cultures of hamster kidney cells HKL infected with the Asibi strain of yellow fever virus. Specific viral antigen was first seen in HKL cells by immunofluorescence at 12 to 15 hours. The amount of antigen increased gradually through 24 hours. Membrane-limited particles containing dense cores were seen at 18 hours in the electron microscope. These particles measured 42 2 millimicrons in diameter. Ultrastructural changes in the HKL cells were limited to a fine vesicular alteration in the endoplasmic reticulum and the appearance of virus particles within vacuoles. The liver cells of cynomolgus monkeys inoculated with yellow fever virus showed a progressive loss of glycogen and a reorganization of the ribosomes into polyribosomes. Characteristic virus particles were not seen and the immunofluorescent observations corroborated the paucity of parenchymal cells containing mature virus.