GENETIC, SEROLOGIC, AND BIOCHEMICAL STUDIES ON VIRAL INFECTION AND LYSOGENIZATION.
Rept. no. 2, Final, 20 Jan 63-19 Jan 64,
SAPPORO MEDICAL COLL (JAPAN)
Pagination or Media Count:
Salmonella phage undergoes a two-way host-controlled viriation as described before. Besides multiplicity activation MA, a thermo-labile factor responsible for phage growth restriction was found. Experiments with P32-labled phages show that the DNA of the restricted phage is degraded rapidly after injection of the nonpermissive host, and that the degradation is prevented by heating the cells prior to infection. MA-responsible, thermolabile, and DNA-destroying factors are suggested to be identical. Chemical, serological, and genetic studies have revealed the followings Salmonella senftenberg 87Aa with O antigen 3 alone has alpha-galactosyl-mannosylrhamnose as serological determinant specificity 3 is determined by mannosyl-rhamnose another cross reaction between El strains and 87Aa is attributed to alpha-galactosyl structure 87Aa is suspected to carry defective prophage lacking conversion genes. Author