PURIFICATION AND LIPID CONTENT OF VEE VIRUS
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The virus of Venezuelan equine encephalitis VEE was purified from chick embryo suspensions by two methods, and the total lipid content and RNA content of the purified virus preparations were compared. The lipid content of VEE virus purified by sucrose gradient centrifugration was much lower 24 per cent than that of VEE virus purified without gradient centrifugation 54. Virus of lower lipid was still fully infectious. With the decrease in lipid content of the virus there was a concomitant increase in RNA content. Electron microscopic examination of purified VEE irus showed that the virus had an average diameter of 68 millimicrons and possessed a center core about 30 millimicrons in diameter.