ON THE GROWTH MECHANISM OF PSITTACOSIS-TRACHOMA VIRUSES IN TISSUE CULTURES
Rept. no. 2 (Final) 5 Feb 1962-4 Feb 1963
KYOTO UNIV (JAPAN)
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Trachoma virus has been successfully adapted to HeLa cell. The virus passes through eclipse phase during the multiplication and seems to arise from the matrix material, therefore there is no immediate morphological continuity between the infected particles and the new generation of the virus. There is no evidence that trachoma viruses multiply by vinary fission which had been supposed so far. It was found that the preparations of meningopneumonitis virus MPV purified by a combination of differential centrifugation, treatments by nucleases and trypsin, and sucrose density gradient centrifugation consisted mostly of elementary bodies of the virus. Chemical analysis of the elementary body showed that it contained approximately equal amount of ribo-and deoxyribonucleic acid.