PURIFICATION OF HISTAMINE SENSITIZING FACTOR OF BORDETELLA PERTUSSIS.
Final rept. no. 2, 1 Oct 62-30 Sep 63,
OSAKA CITY UNIV (JAPAN) SCHOOL OF MEDICINE
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Histamine Sensitizing Factor HSF of Bordetella pertussis was studied. To improve the cultivation method for HSF preparation, growth conditions of B. pertussis were investigated. Ribonucleotides as well as spermine stimulated the growth in a semi-synthetic medium. A new medium suitable for maintenance and surface bulk culture was devised. Intracellular localization of HSF and lethal toxin were studied. HSF was mainly localized in the cell wall prepared by Mickle disintegration, and toxin was found in protoplasm Ribosomes prepared from sonicate contained considerable amount of HSF and toxin. Properties and biological activities of HSF preparation were investigated. Homogeneous preparation of HSF has not yet been obtained. Protective antigen potency against intracellular challenge with B.pertussis was observed in HSF preparation, but conclusion is reserved for further investigation. Author