SEROLOGICAL SURVEY ON THE ANTIBODY LEVEL AGAINST HERPES VIRUS AMONG A POPULATION IN A LIMITED AREA OF TOKYO AND VICINITY.
Final rept., 11 Apr 62-10 Apr 63,
YOKOHAMA MUNICIPAL UNIV (JAPAN)
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Some childrens serums contained comparatively heat-labile complement-fixing antibodies, capable of reacting with herpes V antigen, despite the lack of neutralizing antibodies. A simple method for the serum neutralization with herpes simplex virus was developed utilizing the ability of this virus to form plaques in chick embryo monolayers. A constant virus-varying serum system was adopted. The device was the use of eight small agar cover slips ACS, prepared by punching out of a solidified thin layer of an agar overlay solution, for inoculation f virus-serum mixtures onto a monlayer dish, so that each serum sample could be titrated on one monolayer dish. Serum samples from 39 normal healthy persons were tested by this method and by complement fixation test, and the serum endopoints obtained by the two tests showed a good parallelism. Serum samples collected in clinical wards from patients suffering from keratitis, stomatitis, hepatitis, dermatitis and meningitis were tested for neutralizing antibodies against herpes virus by the above-stated method. Virus isolation was also attempted. Four children showing the symptom of hepatitis all showed positive results. SK strain of herpes simplex virus was further passaged in eggs and clone-purified by plaque isolation in chick embryo fibroblast cultures. Author