Accession Number:

AD0431955

Title:

ACETATE, FORMATE AND RELATED ENZYMES AS FACTORS IN METABOLISM.

Descriptive Note:

Final rept., 1 Nov 61-31 Oct 62,

Corporate Author:

OREGON STATE UNIV CORVALLIS

Personal Author(s):

Report Date:

1962-10-31

Pagination or Media Count:

17.0

Abstract:

Homogenates of the larvae of Phormia regina oxidize formate to carbon dioxide. The formate oxidizing enzyme was purified about eighteen-fold, and simultaneously a similar purification of catalase was obtained. Formate oxidation is competitively inhibited by nitrite and ethanol and is oxidized by a catalase-peroxide complex. Hydrogen peroxide is generated by oxidation of reduced sulfhydryls like glutathione and homocysteine. It is also produced by the oxidation of hypoxanthine by xanthine oxidase, NADH sub 2 and NADPH sub 2 plus plus through flavins, and possibly folate. Thus formate oxidation in Phormia involves coupled reactions between enzyme systems producing hydrogen peroxide except glutathione oxidation if it is a non-enzymatic reaction to form a complex with catalase, which in turn oxidizes formate to carbon dioxide. If Phormia regina is grown on a diet in which carnitine replaces choline, then a new phosphatid, phosphatidyl methylcholine is made. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE