EFFECTS OF BICARBONATE ON GROWTH OF PASTEURELLA PESTIS. I. A DIFFERENTIAL RESPONSE OF VIRULENT AND AVIRULENT CELLS
ARMY BIOLOGICAL LABS FREDERICK MD
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The observation of Delwiche et al that the virulence of broth cultures of Pasteurella pestis aerated at 37 C can be maintained by addition of bicarbonate was confirmed and extended. A differential growth response by virulent and avirulent cells to added bicarbonate under certain conditions was demonstrated. The effect of supplemental bicarbonate on initiation of growth from a virulent inoculum was found to be a stimulation of both virulent and avirulent cells at low levels of added bicarbonate, b depression of both virulent and avirulent cells at higher initial concentrations, or c depression of avirulent mutants with simultaneous stimulation of virulent cells at a narrow range of intermediate levels.