STUDIES ON THE TOXINS OF PSEUDOMONAS COCOVENENANS
Final technical rept., 1 Jan 1963-1 Jan 1964
TECHNISCHE HOGESCHOOL DELFT (NETHERLANDS) BIOCHEMICAL AND BIOPHYSICAL LAB
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Fatal food poisonings have repeatedly occurred amongst the natives of the densely populated parts of Mid-Java in Indonesia. These were caused by the eating of coconut-products bongkrek that had been inoculated with moulds Rhizopus oryzae. A study of the background of these poisonings was made round about 1930. An extensive investigation brought to light the fact that sometimes a bacterium developed instead of the mould with which the defatted coconut was inoculated and this secreted a very active poison. This bongkrekic bacterium was identified as belonging to the genus Pseudomonas and it got the name Pseudomonas cocovenenans. The toxic compound, bongkrekic acid, was isolated from cultures of this microorganism on moist, defatted copra. This isolation was worked out by using extraction procedures followed by thin layer chromatography or liquid-liquid chromatography on Sephadex. The concentration of bongkrekic- acid at the different phases of isolation was determined by measuring the UV- absorption or the antibiotic activity against Cladosporium cucumerinum.