STUDIES ON THE CHANGE OF THE CONCENTRATIONS OF INTERMEDIATES DURING PHOTOSYNTHESIS OF CHLORELLA AND ISOLATED CHLOROPLASTS.
Final rept. no. 1,
TECHNISCHE HOCHSCHULE MUNICH (WEST GERMANY)
Pagination or Media Count:
The chages in the concentrations of intermediates and the distribution of C14 after photosynthesis in C1402 by Chlorella and isolated chloroplasts was measured under different conditions of photosynthesis and under the effect of metabolic inhibitors. The following results were obtained. 1 The changes in the concentrations of intermediates in Chlorella during the transition from light to dark and vice versa are independent of CO2, but dependent on anaerobiosis. 2 In Chlorella photosynthesis is inhibited even by a concentration of monoiodoacetic acid which is 100 times lower than that inhibiting respiration. The beginning of inhibition is indicated by an accumulation of labeled PGA. 3 When Chlorella is illuminated in the presence of KCN, the radioactive PGA formed during a preceding period of photosynthesis in C1402, is still reduced to sugar without a change in the total quantity of PGA in the cell. 4 Arsenate and DNP do not lead to an accumulation of PGA, but do inhibit the formation of RuDP. 5 The distribution of radioactivity on the paper chromatograms showed that ATP and ADP are the first labeled compounds under the two conditions. Author