Accession Number:

AD0425883

Title:

RESEARCH ON PROCEDURES FOR THE LOW-TEMPERATURE PRESERVATION OF BLOOD. THE ANALYTICAL DETERMINATION OF POLYVINYLPYRROLIDONE,

Descriptive Note:

Corporate Author:

UNION CARBIDE CORP TONAWANDA N Y LINDE DIV

Personal Author(s):

Report Date:

1963-12-13

Pagination or Media Count:

39.0

Abstract:

Two methods have been developed for the analytical determination of PVP in serum and urine from experimental dogs. In the iodine titration method, the sample is treated with an iodine solution to form a PVP-I2 complex which is spearated from the solution by centifugation, and then disolved in methyl cellosolve. The iodine is titrated with standard thiosulfate solution. In the second method, the absorption of infrared radiation by PVP is measured at a wavelength of 7.75 microns. The iodine method gives values for PVP concentrations in serum and urine which are accurate within 10, and the infrared method gives similar accuracy for PVP in serum. In the case of urine samples analyzed by the infrared method, creatinine is sometimes administered to the experimental dogs along with PVP. When the creatininePVP weight ratio is less than about 11, the accuracy of the PVP determinations by the infrared method is within about 10, but when the creatininePVP ratio exceeds about 11, the accuracy varies from 10 to 50 as the creatininePVP ratio varies from about 1 to about 100. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE