ATROPINE DETOXICATION DURING HYPOTHERMIA BY THE ISOLATED PERFUSED RAT LIVER.
Final technical rept.
PITTSBURGH UNIV PA SCHOOL OF MEDICINE
Pagination or Media Count:
Considerable information was gained on the metabolism of C14 atropine by the isolated, perfused rat liver under normothermic and hypothermic conditions. The half-life of the drug is prolonged under hypothermic conditions, and the per cent of drug remaining in the plasma 4 hours after administration is greater at the low temperatures. Four hours after administration, the liver perfused at 17.5 C has secreted only 20 as much C14 into the bile as has the liver perfused at 37 C. At 25 C, bile collected 4 hours after drug administration shows about 60 of the amount secreted at normothermic temperatures. At all temperatures, the material appearing in the bile is an altered form of the drug and none of the 5 chromatographically separable compounds is tropic acid. Biliary secretion of atropine metabolites by intact, nephrectomized rats shows good agreement with similar data obtained from the isolated, perfused liver at 37 C and at 25 C. At 17.5 C, the biliary secretion of atropine metabolites may be greater by the perfused preparation than by the intact rat. Preliminary observations on some physiological parameters, bile flow and oxygen utilization, have been made on the perfused liver at 37, 25 and 17.5 degrees C. Author