Accession Number:

AD0419563

Title:

INDUCTION OF HOMOGRAFT TOLERANCE IN SUBLETHALLY X-IRRADIATED ADULT MICE,

Descriptive Note:

Corporate Author:

NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF

Personal Author(s):

Report Date:

1963-08-19

Pagination or Media Count:

12.0

Abstract:

An attempt has been made to achieve homograft tolerance in sublethally irradiated adult mice under conditions which avoid secondary disease. Allogeneic mice which share the same H-2 locus, but differ at other histocompatibility loci, have now been used with some success. A tolerant state has been produced in CBA H-2k mice by first exposing them to sublethal X-radiation 500 rad or urethan-treatment coupled with irradiation, and then injecting C3H H-2k spleen 18 x 10 to the 6th power andor bone marrow 42 x 10 to the 6th power cells within 3 days post irradiation. These mice have retained subsequent C3H skin homografts over 165 days but have rejected BALBc skin grafts H-2d within 33 days. On the other hand, irradiated and urethan-treated controls, uninjected or injected with X-ray inactivated 2000 rad in vitro C3H cells, rejected both C3H and BALBc homografts by 70 days. Similar results, although less definitive, have been obtained using C3H Hd2k or BALBc H-2d as the recipient mice and CBA H-2k or DBA2 H-2d mice respectively, for the donor cells. Such procedures, however, have been unsuccessful in producing homograft tolerance between donor and host F1 hybrid mice which have one H-2 histocompatibility locus in common but differ at the other. The significance of these results is discussed. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE