STUDY OF THE ORGANIC ACIDS IN SERUM, URINE AND BLOOD DIALYSATE OF PATIENTS WITH MEEMIA.
Final technical rept., Jan 59-Dec 62,
YALE UNIV NEW HAVEN CONN
Pagination or Media Count:
The signs and symptoms of uremia appear to be related to the organic anions which accumulate and circulate in body fluids following renal in sufficiency. In uremic patients these anions rise to 10-20 meqL as compared to normal, which is 6. Fractionation of the anions reveal several hundred definitive compounds observed after counter-current fractionation and paper chroma tography. These compounds are demonstrated by their ability to absorb ultraviolet light or to fluoresce. Compounds such as indoles, phenols, substituted hippuric acids have been identified. Some of the latter, such as o-hydroxybenzoylgly cine have been isolated and absolutely identi fied from dialysis fluids obtained remic patients. Techniques have been established for measuring, isolating and identifying minute amounts of compound. Toxicity studies on mice, nephrectomized mice, maturation of erythrocytes, tissue culture growth and protein synthesis have been attempted using compounds isolated from uremic fluid. All studies showed some inhibi tory effects but were erratic and difficult to reproduce. One compound was isolated from more than one patient which consistently inhibited protein synthesis using a purified ribosomal liver mitrochondria system. After repeated se paration by various types of chromatography, in hibitory activity remained. Author