Accession Number:

AD0416643

Title:

ENZYMATICALLY AND PHYSICALLY INDUCED INHERITANCE CHANGES IN BACILLUS SUBTILIS

Descriptive Note:

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s):

Report Date:

1963-08-01

Pagination or Media Count:

40.0

Abstract:

When a culture of Bacillus subtilis strain 168 is treated with a solution of crystalline lysozyme, the bacteria pass through an osmotically sensitive bacillary phase to the protoplast stage. All of the protoplasts can be recovered as L- colony-forming elements on complex or chemically defined osmotically stabilized agar media. Protoplasts or L-bodies can be propagated in the L-state indefinitely or can be mass-reverted to the bacillary state by altering medium in gredients.

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE