HYPOTHALAMIC SECRETORY FACTOR FOR ADRENOCORTICOTROPIC HORMONE (SF-ACTH).
Final Technical rept.,
HARVARD SCHOOL OF DENTAL MEDICINE BOSTON MASS
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Research on the biological assay and purification of the hypothalamic secretory factor for ACTH HSF-ACTH has been described. Extracts of hypothalamic tissue are evaluated by testing for decrease in adrenal ascorbic acid as an indicator of endogenous or exogenous ACTH in three types of rats. Rats anesthetized with pentobarbital are used to estimate proper dose levels and to detect nonspecific stressful contaminants. Rats anesthetized with pentobarbital and treated with morphine, which do not respond to moderate stimulation by nonspecific substances, are used to estimate the amount of HSF-ACTH, but they also respond to ACTH. Hypophysectomized rats are used to estimate the amount of exogenous ACTH that may contaminate the HSF-ACTH preparations. Author