RAPID MICROBIOLOGICAL DETECTION
Quarterly rept. no. 1, 1 May-31 July 1963
RESOURCES RESEARCH INC WASHINGTON DC
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A new tchnique for the bioassay of ATP was developed in which the reaction is allowed to take place on filter paper. A major advantage of this technique is that it allows a rapid con centration of dilute ATP. A similar method which employs glass plates instead of filter paper is under development. Studies were carried out with partially purified preparations of luciferase and luciferin in an effort to under stand, and possibly eliminate the inherent light effect. While the mechanism of the inherent light effect is not yet defined, it appears that an initial one step treatment with calcium phosphate gel eliminates a major part of the inherent light without affecting the enzyme activity to any great extent. The extraction of ATP from E. coli and S. ceravisiae using differ ent solvents is now being studied. Using ex traction with hot water as the basis of com parison, perchloric acid was not as effective while acetone when mixed with water at a ten to one ratio effected a significantly better extraction. Detection of 3,800 yeast cells has been realized. By using methanol extraction it has been possible to obtain a positive response from algae.