RED CELL METABOLISM RELATED TO BLOOD STORAGE.
Progress rept., 1 Nov 61-30 June 63,
LABORATORY FOR COMPARATIVE BIOCHEMISTRY LA JOLLA CALIF
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Red cells from frman blood were found to incorporate C14-labeled adenosine or adenine when inosine was present into ATP. No in corporation was obtained from C14-labeled hypoxanthine, inosine or adenine. A net syn thesis of ATP above the steady-state level could account for the amount of incorporation and it was concluded that the turnover rate of ATP in this period of time was negligible. Further evidence for an active glycogen metabolism in the human red cell has been obtained in the form of a rapid turnover of both the sugar and phosphorus parts of UDPG and glucose-1, 6-diphosphate. A new sugar phosphate has been discovered to be a normal constituent of the human red cell and has been identified tentatively as mannose-1, 6-diphosphate. Of special significance was the finding by isotope analyses that this new com pound is actively integrated with the sugar and phosphorus metabolism of the cell. The phos phorylated metabolites of the rabbit reticulocyte have been analysed and compared with those of the mature erythrocyte. The distinctive features of the glycolytic apparatus of the mature erythrocyte were found in the reticulocyte the principle differences were the presence in the reticulocyte of small quantities of additional uridine, cytidine, and guanosine nucleotides. Author