Accession Number:

AD0408632

Title:

TRYPTOPHANASE-TRYPTOPHAN SYNTHETASE SYSTEMS IN ESCHERICHIA COLI. I. EFFECT OF TRYPTOPHAN AND RELATED COMPOUNDS,

Descriptive Note:

Corporate Author:

MINNESOTA UNIV MINNEAPOLIS

Report Date:

1961-06-14

Pagination or Media Count:

9.0

Abstract:

The effect of tryptophan and related compounds on tryptophanase and tryptophan synthetase formation in Esscherichia coli was determined. Several of these compounds stimulated the formation of tryp tophanase while concomitantly decreasing the production of synthetase. A number of tryptophan analogues were found to inhibit growth. The possible mode of action of these substances was examined further. 5-Hydroxytryptophan greatly inhibited the formation of synthetase and also reduced growth. Its inhibitory action on growth was attributed, at least partially, to the false feedback inhibition of anthranilic acid formation. Trytamine was found to be a potent inhibitor of the activity of synthetase, as well as of the enzymes involved in the synthesis of anthranilic acid from schikimic acid. However, growth reduc tion was only partially reversed by tryptophan. Indole-3-acetic acid and indole-3-propionic acid decreased growth and increased the formation of synthetase six- to eightfold. The action of these compounds was ascribed to their ability to block the endogenous formation of tryptophan. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE