A COMPREHENSIVE STUDY OF VIRAL HEPATITIS
Annual progress rept. 15 July 1962-14 July 1963
JOHNS HOPKINS UNIV BALTIMORE MD DEPT OF PATHOLOGY
Pagination or Media Count:
A study of viral agents associated with infectious hepatitis in man was made. The study of candidate agents was investigated. A major effort was the study of the virus tissue culture cell system under specified conditions utilizing several cloned lines of D6y cells, infective plasma, and cultures of representative agents. Cytopathic effects were obtained only sporatically and unpredictably. The San Carlos 8 agent was found to multiply readily in a variety of human cell cultures. The observation of others that this agent types as an adenovirus type 16 was confirmed but an antiserum produced in this laboratory against the San Carlos agent appeared to show a one-way prime relationship rather than a relationship of identity. CF tests with 10-paired sera from confirmed cases of Korean hepatitis against the San Carlos agent showed positive reactions of 1 in 8 or greater and only one-half the cases and none showed a significant rise in titer. The histopathologic aspects of viral hepatitis are described. Descriptions of the histologic findings in liver needle biopsies on 100 chimpanzees of various ages and sexes on liver needle biopsies from chimpees with so called contact viral hepatitis, and of liver biopsies from human volunteers inoculated with the Parke, Davis agents are described in considerable detail in the main report.
- Medicine and Medical Research